MYD88 ELISA 试剂盒
Quick Overview for MYD88 ELISA 试剂盒 (ABIN5690914)
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See all MYD88 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human MyD88 ELISA Kit.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair recognizes Human MyD88.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA Kit
MYD88 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 人 AA 1-296 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 6 months
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- MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
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别名
- MyD88
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基因ID
- 4615
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UniProt
- Q99836
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途径
- NF-kappaB Signaling, TLR signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
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