PCNA ELISA 试剂盒
-
- 抗原 See all PCNA ELISA试剂盒
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
-
适用
- 人, 大鼠, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Proliferating Cell Nuclear Antigen (PCNA). No significant cross-reactivity or interference between Proliferating Cell Nuclear Antigen (PCNA) and analogues was observed.
- 灵敏度
- 0.054 ng/mL
- Featured
- Discover our best selling PCNA ELISA Kit
- Top Product
- Discover our top product PCNA ELISA Kit
-
Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit
PCNA 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Proliferating Cell Nuclear Antigen (PCNA) ELISA KitPCNA 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Proliferating Cell Nuclear Antigen (PCNA) ELISA KitPCNA 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Proliferating Cell Nuclear Antigen (PCNA) ELISA KitPCNA 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Proliferating Cell Nuclear Antigen (PCNA) ELISA KitPCNA 适用: 兔 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proliferating Cell Nuclear Antigen (PCNA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proliferating Cell Nuclear Antigen (PCNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proliferating Cell Nuclear Antigen (PCNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proliferating Cell Nuclear Antigen (PCNA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proliferating Cell Nuclear Antigen (PCNA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proliferating Cell Nuclear Antigen (PCNA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all PCNA ELISA试剂盒
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- 别名
- Proliferating Cell Nuclear Antigen (PCNA 产品)
- 别名
- PCNAR ELISA Kit, Pcna/cyclin ELISA Kit, pcna ELISA Kit, AO090003000846 ELISA Kit, 53/13 ELISA Kit, CG9193 ELISA Kit, DmPCNA ELISA Kit, Dmel\\CG9193 ELISA Kit, MUS209 ELISA Kit, Mus209 ELISA Kit, PCNA ELISA Kit, PCNA/mus209 ELISA Kit, Pcna ELISA Kit, dPCNA ELISA Kit, l(2)02448 ELISA Kit, l(2)56F ELISA Kit, l(2)56Fa ELISA Kit, l(2)s1534 ELISA Kit, cb16 ELISA Kit, fb36g03 ELISA Kit, wu:fa28e03 ELISA Kit, wu:fb36g03 ELISA Kit, pcna-A ELISA Kit, proliferating cell nuclear antigen ELISA Kit, DNA polymerase III sliding clamp ELISA Kit, Proliferating cell nuclear antigen ELISA Kit, Proliferating cell nuclear antigen-like ELISA Kit, proliferating cell nuclear antigen L homeolog ELISA Kit, proliferating cell nuclear antigen 1 ELISA Kit, PCNA ELISA Kit, Pcna ELISA Kit, pcna ELISA Kit, MMP_RS08810 ELISA Kit, ANI_1_512014 ELISA Kit, AOR_1_1500154 ELISA Kit, MGC53867 ELISA Kit, pcna.L ELISA Kit, pcna1 ELISA Kit, LOC106420145 ELISA Kit, pcn1 ELISA Kit
- 背景
-
Gene Name: Proliferating Cell Nuclear Antigen
Gene Aliases: Cyclin
- 途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
-
