PARP1 ELISA 试剂盒
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- 抗原 See all PARP1 ELISA试剂盒
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.781 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.781 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Poly ADP Ribose Polymerase (PARP). No significant cross-reactivity or interference between Poly ADP Ribose Polymerase (PARP) and analogues was observed.
- 灵敏度
- 0.28 ng/mL
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Poly (ADP-Ribose) Polymerase 1 (PARP1) ELISA Kit
PARP1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Poly (ADP-Ribose) Polymerase 1 (PARP1) ELISA KitPARP1 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Poly (ADP-Ribose) Polymerase 1 (PARP1) ELISA KitPARP1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poly ADP Ribose Polymerase (PARP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poly ADP Ribose Polymerase (PARP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poly ADP Ribose Polymerase (PARP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poly ADP Ribose Polymerase (PARP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PARP1 ELISA试剂盒
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- 别名
- Poly ADP Ribose Polymerase (PARP1 产品)
- 别名
- ADPRT ELISA Kit, ADPRT 1 ELISA Kit, ADPRT1 ELISA Kit, ARTD1 ELISA Kit, PARP ELISA Kit, PARP-1 ELISA Kit, PPOL ELISA Kit, pADPRT-1 ELISA Kit, Adprt ELISA Kit, Parp-1 ELISA Kit, 5830444G22Rik ELISA Kit, AI893648 ELISA Kit, Adprp ELISA Kit, Adprt1 ELISA Kit, C80510 ELISA Kit, parp-1 ELISA Kit, sPARP-1 ELISA Kit, BEST:LD21673 ELISA Kit, CG17685 ELISA Kit, CG17696 ELISA Kit, CG17718 ELISA Kit, CG40411 ELISA Kit, D.PARP ELISA Kit, Dm.pARTa ELISA Kit, Dmel\\CG40411 ELISA Kit, LD21673.3prime ELISA Kit, PARP1 ELISA Kit, dPARP ELISA Kit, parp ELISA Kit, adprt1 ELISA Kit, padprt-1 ELISA Kit, ppol ELISA Kit, si:dkey-206f10.3 ELISA Kit, wu:fc60f12 ELISA Kit, zgc:110092 ELISA Kit, poly(ADP-ribose) polymerase 1 ELISA Kit, poly (ADP-ribose) polymerase 1 ELISA Kit, poly (ADP-ribose) polymerase family, member 1 ELISA Kit, Poly-(ADP-ribose) polymerase ELISA Kit, poly(ADP-ribose) polymerase 1 L homeolog ELISA Kit, PARP1 ELISA Kit, Parp1 ELISA Kit, Parp ELISA Kit, parp1.L ELISA Kit, parp1 ELISA Kit
- 背景
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Gene Name: Poly ADP Ribose Polymerase
Gene Aliases: PARP1, ADPRT, ADPRT1, PPOL, pADPRT-1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD(+) ADP-ribosyltransferase 1
- 基因ID
- 142
- UniProt
- P09874
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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