PAI1 CLIA Kit
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- 抗原 See all PAI1 (SERPINE1) CLIA Kits
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
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适用
- 大鼠
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 312.5 pg/mL - 20000 pg/mL
- 最低检测浓度
- 312.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator Inhibitor 1 (PAI1). No significant cross-reactivity or interference between Plasminogen Activator Inhibitor 1 (PAI1) and analogues was observed.
- 灵敏度
- 11.5 pg/mL
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Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA Kit
SERPINE1 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 马 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 兔 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Plasminogen Activator Inhibitor 1 (PAI1) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PAI1 (SERPINE1) CLIA Kits
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
- 别名
- Plasminogen Activator Inhibitor 1 (SERPINE1 产品)
- 别名
- PAI CLIA Kit, PAI-1 CLIA Kit, PAI1 CLIA Kit, PLANH1 CLIA Kit, SERPINE1 CLIA Kit, si:ch211-138a11.1 CLIA Kit, Planh1 CLIA Kit, PAI1A CLIA Kit, Pai1 CLIA Kit, Pai1aa CLIA Kit, Planh CLIA Kit, RATPAI1A CLIA Kit, serpin family E member 1 CLIA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog CLIA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 CLIA Kit, serine (or cysteine) peptidase inhibitor, clade E, member 1 CLIA Kit, SERPINE1 CLIA Kit, serpine1.L CLIA Kit, serpine1 CLIA Kit, Serpine1 CLIA Kit
- 背景
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Gene Name: Plasminogen Activator Inhibitor 1
Gene Aliases: SERPINE1, PLANH1, Serpin Peptidase Inhibitor Clade E Member 1, Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1, Endothelial plasminogen activator inhibitor
- 基因ID
- 24617
- UniProt
- P20961
- 途径
- p53 Pathway, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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