Phospholipase C gamma 1 ELISA 试剂盒
-
- 抗原 See all Phospholipase C gamma 1 (PLCG1) ELISA试剂盒
- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Phospholipase C Gamma 1 (PLCg1). No significant cross-reactivity or interference between Phospholipase C Gamma 1 (PLCg1) and analogues was observed.
- 灵敏度
- 0.057 ng/mL
- Featured
- Discover our best selling PLCG1 ELISA Kit
- Top Product
- Discover our top product PLCG1 ELISA Kit
-
phospholipase C, gamma 1 (PLCG1) ELISA Kit
PLCG1 适用: 人, 小鼠, 大鼠 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
phospholipase C, gamma 1 (PLCG1) ELISA KitPLCG1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
phospholipase C, gamma 1 (PLCG1) ELISA KitPLCG1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
phospholipase C, gamma 1 (PLCG1) ELISA KitPLCG1 适用: 大鼠 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Gamma 1 (PLCg1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase C Gamma 1 (PLCg1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Gamma 1 (PLCg1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase C Gamma 1 (PLCg1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase C Gamma 1 (PLCg1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase C Gamma 1 (PLCg1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all Phospholipase C gamma 1 (PLCG1) ELISA试剂盒
- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
- 别名
- Phospholipase C Gamma 1 (PLCG1 产品)
- 别名
- NCKAP3 ELISA Kit, PLC-II ELISA Kit, PLC1 ELISA Kit, PLC148 ELISA Kit, PLCgamma1 ELISA Kit, plc1 ELISA Kit, nckap3 ELISA Kit, plc-ii ELISA Kit, plc148 ELISA Kit, plcgamma1 ELISA Kit, PPLCA ELISA Kit, AI894140 ELISA Kit, Cded ELISA Kit, Plc-1 ELISA Kit, Plc-gamma1 ELISA Kit, Plcg-1 ELISA Kit, fj46f04 ELISA Kit, wu:fj46f04 ELISA Kit, wu:fj57a05 ELISA Kit, phospholipase C gamma 1 ELISA Kit, phospholipase C gamma 1 L homeolog ELISA Kit, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 ELISA Kit, phospholipase C, gamma 1 ELISA Kit, PLCG1 ELISA Kit, plcg1.L ELISA Kit, LOC100633163 ELISA Kit, Plcg1 ELISA Kit, plcg1 ELISA Kit
- 背景
-
Gene Name: Phospholipase C Gamma 1
Gene Aliases: NCKAP3, PLC-G1, PLC-II, PLC1, PLC148, PLCgamma1, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, Phosphoinositide phospholipase C-gamma-1
- 基因ID
- 5335
- UniProt
- P19174
- 途径
- RTK signaling, WNT signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling
-
