MYD88 ELISA 试剂盒
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- 抗原 See all MYD88 ELISA试剂盒
- MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Myeloid Differentiation Factor 88 (MyD88). No significant cross-reactivity or interference between Myeloid Differentiation Factor 88 (MyD88) and analogues was observed.
- 灵敏度
- 27 pg/mL
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Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA Kit
MYD88 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA KitMYD88 适用: 人 AA 1-296 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Differentiation Factor 88 (MyD88). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Differentiation Factor 88 (MyD88). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Differentiation Factor 88 (MyD88), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Differentiation Factor 88 (MyD88) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Differentiation Factor 88 (MyD88) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Differentiation Factor 88 (MyD88) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MYD88 ELISA试剂盒
- MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
- 别名
- Myeloid Differentiation Factor 88 (MYD88 产品)
- 别名
- MYD88D ELISA Kit, XMyD88 ELISA Kit, myd88d ELISA Kit, MGC84928 ELISA Kit, CG2078 ELISA Kit, DMMYD88 ELISA Kit, DmMyD88 ELISA Kit, DmMyd88 ELISA Kit, Dmel\\CG2078 ELISA Kit, EP(2)2535 ELISA Kit, Kra ELISA Kit, LD20892 ELISA Kit, MYD88 ELISA Kit, MyD88 ELISA Kit, Myd88F ELISA Kit, dMyD88 ELISA Kit, dMyd88 ELISA Kit, kra ELISA Kit, myd88 ELISA Kit, zgc:103541 ELISA Kit, GB12344 ELISA Kit, NV10640 ELISA Kit, myd88-a ELISA Kit, myd88-b ELISA Kit, myeloid differentiation primary response 88 ELISA Kit, myeloid differentiation primary response 88 S homeolog ELISA Kit, myeloid differentiation primary response gene 88 ELISA Kit, CG2078 gene product from transcript CG2078-RB ELISA Kit, myeloid differentiation primary response protein MyD88-A ELISA Kit, myeloid differentiation primary response protein MyD88 ELISA Kit, myeloid differentiation factor 88 ELISA Kit, myeloid differentiation primary response 88 L homeolog ELISA Kit, MYD88 ELISA Kit, myd88.S ELISA Kit, Myd88 ELISA Kit, myd88 ELISA Kit, LOC413194 ELISA Kit, LOC100118553 ELISA Kit, LOC575153 ELISA Kit, myd88.L ELISA Kit
- 背景
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Gene Name: Myeloid Differentiation Factor 88
Gene Aliases: Myeloid Differentiation Primary Response Gene 88
- 基因ID
- 301059
- UniProt
- Q6Y1S1
- 途径
- NF-kappaB Signaling, TLR signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
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