DPP4 ELISA 试剂盒
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- 抗原 See all DPP4 ELISA试剂盒
- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.625 ng/mL - 40 ng/mL
- 最低检测浓度
- 0.625 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase IV (DPP4). No significant cross-reactivity or interference between Dipeptidyl Peptidase IV (DPP4) and analogues was observed.
- 灵敏度
- 0.256 ng/mL
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Dipeptidyl-Peptidase 4 (DPP4) ELISA Kit
DPP4 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 AA 34-766 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 小鼠 AA 29-760 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 犬 Colorimetric Competition ELISA 50-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase IV (DPP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase IV (DPP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase IV (DPP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase IV (DPP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidyl Peptidase IV (DPP4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidyl Peptidase IV (DPP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all DPP4 ELISA试剂盒
- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
- 别名
- Dipeptidyl Peptidase IV (DPP4 产品)
- 别名
- ADABP ELISA Kit, ADCP2 ELISA Kit, CD26 ELISA Kit, DPPIV ELISA Kit, TP103 ELISA Kit, MGC81966 ELISA Kit, MOP9.8 ELISA Kit, MOP9_8 ELISA Kit, DPP4 ELISA Kit, si:ch73-2d23.3 ELISA Kit, Cd26 ELISA Kit, Dpp-4 ELISA Kit, THAM ELISA Kit, dipeptidyl peptidase 4 ELISA Kit, dipeptidyl-peptidase 4 S homeolog ELISA Kit, prolyl oligopeptidase family protein ELISA Kit, dipeptidyl-peptidase 4 ELISA Kit, dipeptidylpeptidase 4 ELISA Kit, DPP4 ELISA Kit, dpp4.S ELISA Kit, AT5G24260 ELISA Kit, dpp4 ELISA Kit, Dpp4 ELISA Kit
- 背景
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Gene Name: Dipeptidyl Peptidase IV
Gene Aliases: CD26, ADA, DPPIV, DPP-IV, ADABP, ADCP2, TP103, Adenosine Deaminase Complexing Protein 2, T-cell activation antigen CD26
- 基因ID
- 25253
- UniProt
- P14740
- 途径
- Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity
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