p21 ELISA 试剂盒
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- 抗原 See all p21 (CDKN1A) ELISA试剂盒
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase Inhibitor 1A (CDKN1A). No significant cross-reactivity or interference between Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) and analogues was observed.
- 灵敏度
- 0.059 ng/mL
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Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA Kit
CDKN1A 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: Cow Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase Inhibitor 1A (CDKN1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all p21 (CDKN1A) ELISA试剂盒
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- 别名
- Cyclin Dependent Kinase Inhibitor 1A (CDKN1A 产品)
- 别名
- CCND1 ELISA Kit, CDKN1A ELISA Kit, CAP20 ELISA Kit, CDKN1 ELISA Kit, CIP1 ELISA Kit, MDA-6 ELISA Kit, P21 ELISA Kit, SDI1 ELISA Kit, WAF1 ELISA Kit, p21CIP1 ELISA Kit, CDKI ELISA Kit, Cdkn1 ELISA Kit, Waf1 ELISA Kit, mda6 ELISA Kit, p21Cip1 ELISA Kit, p21WAF ELISA Kit, Cip1 ELISA Kit, UV96 ELISA Kit, p21 ELISA Kit, cip1 ELISA Kit, p16Xic2 ELISA Kit, sdi1 ELISA Kit, waf1 ELISA Kit, cap20 ELISA Kit, cdkn1 ELISA Kit, mda-6 ELISA Kit, p21cip1 ELISA Kit, si:ch1073-48m5.2 ELISA Kit, KRAS2 ELISA Kit, p21ras ELISA Kit, cyclin D1 ELISA Kit, cyclin dependent kinase inhibitor 1A ELISA Kit, cyclin-dependent kinase inhibitor 1A (P21) ELISA Kit, cyclin-dependent kinase inhibitor 1A ELISA Kit, cyclin-dependent kinase inhibitor 1A L homeolog ELISA Kit, cyclin-dependent kinase inhibitor 1 ELISA Kit, KRAS proto-oncogene, GTPase ELISA Kit, CCND1 ELISA Kit, CDKN1A ELISA Kit, Cdkn1a ELISA Kit, cdkn1a.L ELISA Kit, cki1 ELISA Kit, cdkn1a ELISA Kit, KRAS ELISA Kit
- 背景
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Gene Name: Cyclin Dependent Kinase Inhibitor 1A
Gene Aliases: WAF1, p21, CIP1, p21WAF1, CAP20, CDKN1, MDA-6, SDI1, p21CIP1, CDK-Interacting Protein 1, Melanoma differentiation-associated protein 6
- 基因ID
- 114851
- UniProt
- Q64315
- 途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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