APAF1 CLIA Kit
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- 抗原 See all APAF1 CLIA Kits
- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Apoptotic Peptidase Activating Factor 1 (APAF1). No significant cross-reactivity or interference between Apoptotic Peptidase Activating Factor 1 (APAF1) and analogues was observed.
- 灵敏度
- 6.27 pg/mL
- Top Product
- Discover our top product APAF1 ELISA Kit
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Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit
APAF1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 适用: 小鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Lysate, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apoptotic Peptidase Activating Factor 1 (APAF1) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all APAF1 CLIA Kits
- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
- 别名
- Apoptotic Peptidase Activating Factor 1 (APAF1 产品)
- 别名
- APAF1 CLIA Kit, ARK CLIA Kit, Apaf-1 CLIA Kit, Apaf1 CLIA Kit, CG6829 CLIA Kit, D-Apaf-1 CLIA Kit, DARK CLIA Kit, Dapaf-1 CLIA Kit, Dapaf-1/HAC-1 CLIA Kit, Dark CLIA Kit, Dark/Apaf-I CLIA Kit, Dark/Dapaf-1/HAC1 CLIA Kit, Dark/Hac-1/dApaf-1 CLIA Kit, Dark/Hac-1/dApaf1 CLIA Kit, Dmel\\CG6829 CLIA Kit, Hac-1 CLIA Kit, Hac-1/Dark CLIA Kit, Hac1 CLIA Kit, T1 CLIA Kit, anon-53Fa CLIA Kit, apaf-1 CLIA Kit, apaf1 CLIA Kit, arc CLIA Kit, ark CLIA Kit, dAPAF-1 CLIA Kit, dApaf-1 CLIA Kit, dApaf-1/DARK/HAC-1 CLIA Kit, dApaf1 CLIA Kit, dArk CLIA Kit, dapaf CLIA Kit, dapaf-1 CLIA Kit, dapaf-1L CLIA Kit, dapaf-1S CLIA Kit, dark CLIA Kit, dark/dapaf-1/hac-1 CLIA Kit, dark/hac-1/dapaf-1 CLIA Kit, hac-1 CLIA Kit, hac1 CLIA Kit, l(2)SH0173 CLIA Kit, APAF-1 CLIA Kit, CED4 CLIA Kit, 6230400I06Rik CLIA Kit, Apaf1l CLIA Kit, fog CLIA Kit, mKIAA0413 CLIA Kit, zgc:136543 CLIA Kit, Death-associated APAF1-related killer CLIA Kit, apoptotic peptidase activating factor 1 L homeolog CLIA Kit, apoptotic peptidase activating factor 1 CLIA Kit, Dark CLIA Kit, apaf1.L CLIA Kit, apaf1 CLIA Kit, APAF1 CLIA Kit, Apaf1 CLIA Kit
- 背景
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Gene Name: Apoptotic Peptidase Activating Factor 1
Gene Aliases: CED4, Apoptotic Protease Activating Factor
- 基因ID
- 317
- UniProt
- O14727
- 途径
- p53 Pathway, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
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