EGF ELISA 试剂盒
Quick Overview for EGF ELISA 试剂盒 (ABIN5067954)
抗原
See all EGF ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- The Human Epidermal Growth Factor (Hu EGF) ELISA is to be used for the in vitro quantitative determination of human EGF in human serum, plasma, buffered solution, cell culture medium or urine. The assay will recognize both natural and recombinant human EGF.
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Analytical Method
- Quantitative
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交叉反应 （详细）
- Calculated cross reactivity: Hu
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灵敏度
- > 1 pg/mL
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产品特性
- Epidermal Growth Factor, Human (EGF) ELISA Kit
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组件
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- * Hu EGF Standard, purified recombinant Hu EGF expressed in E. coli. Refer to vial label for quantity and reconstitution volume. 2vials, 4 vials
- Standard Diluent Buffer 15mM sodium azide 1x25ml, 2x25ml
- Plate Covers, adhesive strips.
- Hu EGF Antibody-Coated Wells, 96 wells per plate. 1plate, 2plates
- Hu EGF Biotin Conjugate (Biotin-labeled anti-EGF). Contains 15 mM sodium azide, 1x11ml, 2x11ml
- Streptavidin-Peroxidase (HRP), (100x) concentrate. Contains 3.3 mM thymol, 1x0.125ml, 2x125ml
- Streptavidin-Peroxidase (HRP) Diluent. Contains 3.3mM thymol, 1x25ml
- Wash Buffer Concentrate (25x), 1x100ml
- Stabilized Chromogen, Tetramethylbenzidine (TMB) 1x25ml
- Stop Solution 1x25ml
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Epidermal Growth Factor (EGF) ELISA Kit
EGF 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 人 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Epidermal Growth Factor (EGF) ELISA KitWide Range EGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 小鸡 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 大鼠 AA 974-1026 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 人 AA 971-1023 Colorimetric Sandwich ELISA 4.7-300 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
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板类型
- Pre-coated
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实验流程
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Principle:
- The human EGF kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A polyclonal antibody specific for human EGF has been coated onto the wells of the microtiter
- strips provided. Samples, including standards of known human EGF content, control specimens, and unknowns, are pipetted into these wells. During the first incubation, the Hu EGF antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for Hu EGF is added. During the second incubation, this antibody binds to the immobilized Hu EGF captured during the first incubation. After removal of excess second antibody, Streptavidin-Peroxidase enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a third incubation and washing to
- remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu EGF present in the original specimen.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C Do not freeze
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- EGF (Epidermal Growth Factor (EGF))
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别名
- Epidermal Growth Factor
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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