IL12 ELISA 试剂盒
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Quick Overview for IL12 ELISA 试剂盒 (ABIN5067892)
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See all IL12 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- For the quantitative determination of human interleukin 12 IL-12) concentrations in cell culture supernates, serum, plasma, and urine. Recognizes native and recombinant human Il-12
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Analytical Method
- Quantitative
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交叉反应 （详细）
- Calculated cross reactivity: Hu
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灵敏度
- 5 pg/mL
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产品特性
- Interleukin 12 (IL-12), ELISA Kit (Human) (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T cell Stimulating Factor, TSF)
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组件
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- IL-12 Microplate: 96 well polystyrene microplate (12 strips of 8 wells) coated with a murine monoclonal antibody against IL-12.
- IL-12 Conjugate: 21ml of polyclonal antibody against IL-12 conjugated to horseradish peroxidase, with preservative.
- IL-12 Standard: 2.5ng of recombinant human IL-12 in a buffered protein base with preservative, lyophilized.
- Assay Diluent R 6ml of a buffered protein base with preservative. For use with cell culture supernate/serum/plasma samples.
- Assay Diluent R 6ml of a buffered protein base with preservative. For use with urine samples.
- Calibrator Diluent R Concentrate (5X): 21ml of a buffered protein base with preservative.
- Wash Buffer Concentrate: 21ml of a 25-fold concentrated solution of buffered surfactant with preservative.
- Color Reagent A: 12.5ml of stabilized hydrogen peroxide.
- Color Reagent B: 12.5ml of stabilized chromogen (tetramethylbenzidine).
- Stop Solution: 6ml of 2 N sulfuric acid.
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Interleukin 12 (IL12) ELISA Kit
IL12 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 小鸡 Colorimetric Competition ELISA 0.78 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle of the Assay:
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL- 12 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-12 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- IL12 (Interleukin 12 (IL12))
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别名
- Interleukin 12 (IL-12)
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途径
- JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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