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EGF ELISA 试剂盒

EGF 适用: 人 Colorimetric Sandwich ELISA 3.9-250 pg/mL Plasma, Serum
产品编号 ABIN5026920
发货至: 中国
  • 抗原 See all EGF ELISA试剂盒
    EGF (Epidermal Growth Factor (EGF))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    3.9-250 pg/mL
    最低检测浓度
    3.9 pg/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of Human EGF concentrations in serum, plasma and other biological fluids.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    灵敏度
    < 2.34 pg/mL
    组件
    plate, standard, diluent, antibodies
    试剂未包括
    pipettes, tubes, reader, incubator
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    Discover our top product EGF ELISA Kit
  • 样本量
    100 μL
    实验时间
    3 - 4 h
    板类型
    Pre-coated
    实验流程
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human EGF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human EGF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EGF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human EGF can be calculated using the standard curve.
    限制
    仅限研究用
  • 注意事项
    protect your eyes
    储存条件
    4 °C
  • 抗原 See all EGF ELISA试剂盒
    EGF (Epidermal Growth Factor (EGF))
    别名
    EGF (Epidermal Growth Factor) (EGF 产品)
    别名
    HOMG4 ELISA Kit, URG ELISA Kit, AI790464 ELISA Kit, CEGF ELISA Kit, epidermal growth factor ELISA Kit, pro-epidermal growth factor ELISA Kit, EGF ELISA Kit, egf ELISA Kit, CpipJ_CPIJ020278 ELISA Kit, Egf ELISA Kit
    途径
    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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