CD40 Ligand ELISA 试剂盒
Quick Overview for CD40 Ligand ELISA 试剂盒 (ABIN4987074)
抗原
See all CD40 Ligand (CD40LG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156.25 pg/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Human sCD40L Ligand
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灵敏度
- 40 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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CD40 Ligand (CD40LG) ELISA Kit
CD40LG 适用: 小鼠 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 人 AA 108-261 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- CD40 Ligand (CD40LG)
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别名
- sCD40L
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背景
- CD40 Ligand (CD40L), also known as CD154, gp39, TNFSF5, TRAP (TNF-Related Activation Protein) or TBAM (T-cell B-cell Activating Molecule), is a multifunctional ligand in the TNF superfamily (1-4). Interaction between CD40 and CD40L is critical to the control of thymus-dependent humoral immunity and cell-mediated immune responses (5-10). The major component of the contact-dependent signal leading to B cell activation is CD40L. CD40L stimulates B cell secretion of immunoglobulin isotypes in the presence of cytokines.CD40L is a 39 kDa, 261 amino acid (aa) glycoprotein that can form homotrimers typical of other TNFSF members (1-4, 11, 12). Proteolytic cleavage can also produce 15-18 kDa soluble forms of CD40L (13, 14). Activated T cells and platelets express both a membrane-associated and a soluble form of CD40L (sCD40L) (13, 15, 16). Platelet activation during plasma and serum sample preparation can result in artificially elevated sCD40L levels (17-20). Conversely, serum samples stored above 2-8
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途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原 See all CD40 Ligand (CD40LG) ELISA试剂盒
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