PARP1 ELISA 试剂盒
Quick Overview for PARP1 ELISA 试剂盒 (ABIN4886363)
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适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human Cleaved PARP (D214/G215) and PARP ELISA Kit. This ELISA is for measuring cleaved PARP (Asp-214/Gly-215) as well as PARP in human cell lysates.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibody pair provided in this kit recognizes human PARP cleaved at sites aspartic acid-214 and glycine-215.
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产品特性
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- Rapidly measures cleaved protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-cleaved Antibody
- Anti-pan Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Poly (ADP-Ribose) Polymerase 1 (PARP1) ELISA Kit
PARP1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Poly (ADP-Ribose) Polymerase 1 (PARP1) ELISA KitPARP1 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Poly (ADP-Ribose) Polymerase 1 (PARP1) ELISA KitPARP1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at -20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.
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有效期
- 6 months
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- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
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别名
- PARP1
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基因ID
- 142
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UniProt
- P09874
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途径
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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