MAPK14 ELISA 试剂盒
Quick Overview for MAPK14 ELISA 试剂盒 (ABIN4886228)
抗原
See all MAPK14 ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- Custom Human Phosphotyrosine P38 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine P38 in lysate samples.
-
Analytical Method
- Semi-Quantitative
-
特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-P38.
-
产品特性
-
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
-
-
Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA Kit
MAPK14 适用: 人, 大鼠, 小鼠 pThr180 Colorimetric Cell ELISA Cell Culture Cells
Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA KitMAPK14 适用: 人, 大鼠, 小鼠 pTyr182 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA KitMAPK14 适用: 人, 大鼠, 小鼠 pTyr182 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA KitMAPK14 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA KitMAPK14 适用: 小鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA KitMAPK14 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA KitMAPK14 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA KitMAPK14 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
-
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
-
有效期
- 6 months
-
-
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
-
别名
- p38
-
基因ID
- 1432
-
UniProt
- Q16539
-
途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins
抗原 See all MAPK14 ELISA试剂盒
-
