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CD59 ELISA 试剂盒

CD59 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4885162
发货至: 中国
  • 抗原 See all CD59 ELISA试剂盒
    CD59
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human CD59 SpeedELISA Kit.
    品牌
    SpeedELISA
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    产品特性
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all CD59 ELISA试剂盒
    CD59
    别名
    CD59 (CD59 产品)
    别名
    16.3A5 ELISA Kit, 1F5 ELISA Kit, EJ16 ELISA Kit, EJ30 ELISA Kit, EL32 ELISA Kit, G344 ELISA Kit, HRF-20 ELISA Kit, HRF20 ELISA Kit, MAC-IP ELISA Kit, MACIF ELISA Kit, MEM43 ELISA Kit, MIC11 ELISA Kit, MIN1 ELISA Kit, MIN2 ELISA Kit, MIN3 ELISA Kit, MIRL ELISA Kit, MSK21 ELISA Kit, p18-20 ELISA Kit, Cd59a ELISA Kit, Cd59b ELISA Kit, MACIP ELISA Kit, CD59 molecule (CD59 blood group) ELISA Kit, CD59 molecule ELISA Kit, CD59 ELISA Kit, Cd59 ELISA Kit
    基因ID
    966
    UniProt
    P13987
    途径
    Complement System
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