CD59 ELISA 试剂盒
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Quick Overview for CD59 ELISA 试剂盒 (ABIN8056011)
抗原
See all CD59 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich Human CD59 ELISA Kit to quantitate Human CD59 in cell culture supernatants, serum.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: L26-N102Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 4 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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CD59 (CD59) ELISA Kit
CD59 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Lysate, Plasma, Serum
CD59 (CD59) ELISA KitCD59 适用: 人 Colorimetric Sandwich ELISA 2.35 ng/mL - 150 ng/mL Plasma, Serum, Tissue Homogenate, Urine
CD59 (CD59) ELISA KitCD59 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
CD59 (CD59) ELISA KitCD59 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD59 (CD59) ELISA KitCD59 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Urine
CD59 (CD59) ELISA KitCD59 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Lysate, Plasma, Serum
CD59 (CD59) ELISA KitCD59 适用: 人 Colorimetric Sandwich ELISA 40-10.000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD59 (CD59) ELISA KitCD59 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 2.6%, 5.9%, 6.0%
Inter-Assay Precision (CV%): 4.4%, 4.0%, 7.7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- CD59
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别名
- CD59
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背景
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Background: CD59 glycoprotein, also known as MAC-inhibitory protein (MAC-IP), membrane inhibitor of reactive lysis (MIRL), or protectin, is a protein that in humans is encoded by the CD59 gene. This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
Gene Full Name: CD59 Molecule (CD59 blood group)
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基因ID
- 966
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UniProt
- P13987
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途径
- Complement System
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