SHP1 ELISA 试剂盒
This 人 SHP1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 SHP1.
Quick Overview for SHP1 ELISA 试剂盒 (ABIN4884518)
抗原
See all SHP1 (PTPN6) ELISA试剂盒
SHP1 (PTPN6)
(Protein-tyrosine Phosphatase 1C (PTPN6))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
-
-
原理
- Custom Human SHP-1 ELISA Kit.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair recognizes .
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Protein-tyrosine Phosphatase 1C (PTPN6) ELISA Kit
PTPN6 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
-
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
有效期
- 6 months
-
-
- SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))
-
别名
- SHP-1
-
基因ID
- 5777
-
UniProt
- P29350
-
途径
- JAK/STAT Signaling, TCR Signaling, TLR signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling
抗原 See all SHP1 (PTPN6) ELISA试剂盒
-
You are here:
