IL-10 ELISA 试剂盒
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北京 101111
Quick Overview for IL-10 ELISA 试剂盒 (ABIN455521)
抗原
See all IL-10 (IL10) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of mouse Interleukin 10,IL-10 concentrations in cell culture supernates, serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay recognizes recombinant and natural mouse IL-10.
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交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
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灵敏度
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< 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. -
产品特性
- Mus musculus,Mouse,Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,Il10,Il-10
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Interleukin 10 (IL10) ELISA Kit
IL10 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 大鼠 AA 19-178 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 小鼠 AA 19-178 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 AA 19-178 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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: "Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages." in: The Journal of pharmacology and experimental therapeutics, Vol. 336, Issue 2, pp. 381-90, (2011) (PubMed).
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- Il10
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背景
- Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 cytokine family. This family currently comprises IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. All known IL-10 family members are secreted -helical proteins. IL-10 is a secreted, possibly glycosylated, polypeptide with a molecular weight of 18 kDa. Based on human studies, IL-10 is likely to circulate as a nondisulfide-linked homodimer. IL-10 is synthesized as a 181 amino acid (aa) precursor with a 19 aa signal sequence and a 162 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which likely form two intra-chain disulfide bridges. Mature rat IL-10 shows 76%, 73%, 72%, 80%, 86%, 85% and 76% aa identity to guinea pig, mouse, human, feline and equine IL-10, respectively. Upon activation, mammalian cells known to secrete IL-10 include NK cells, cytotoxic CD8+ T cells secreting Th2-like cytokines, CD4+CD45RA- (memory) Th1 and Th2 cells, macrophages, monocytes, CD5+ and CD5- B cells, dendritic cells, hepatic stellate (Ito) cells, keratinocytes, melanoma cells, mast cells, placental cytotrophoblasts, and fetal erythroblasts.
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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