PAI1 ELISA 试剂盒
(6 references)-
- 抗原 See all PAI1 (SERPINE1) ELISA试剂盒
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
- 抗原表位
- AA 22-402
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PAI-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: sf21
Immunogen sequence: S22-P402 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: sf21
Immunogen sequence: S22-P402 - Featured
- Discover our best selling SERPINE1 ELISA Kit
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- Discover our top product SERPINE1 ELISA Kit
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Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA Kit
SERPINE1 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 马 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 兔 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the serpin family.
Tissue Specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
- 板类型
- Pre-coated
- 实验流程
- human PAI-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PAI-1 has been precoated onto 96-well plates. Standards(sf21, S22-P402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PAI-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PAI-1 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PAI-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum or plasma(heparin, EDTA, citrate ) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PAI-1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 1539, Standard deviation: 76.95, CV(%): 5
- Sample 2: n=16, Mean(pg/ml): 3736, Standard deviation: 235.4, CV(%): 6.3
- Sample 3: n=16, Mean(pg/ml): 6234, Standard deviation: 405.21, CV(%): 6.5,
- Sample 1: n=24, Mean(pg/ml): 1637, Standard deviation: 104.8, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 3914, Standard deviation: 293.55, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 7335, Standard deviation: 601.47, CV(%): 8.2
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).
: "Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling." in: Cell death & disease, Vol. 9, Issue 2, pp. 122, (2018) (PubMed).
: "Associations between homocysteine metabolism related SNPs and carotid intima-media thickness: a Chinese sib pair study." in: Journal of thrombosis and thrombolysis, Vol. 43, Issue 3, pp. 401-410, (2017) (PubMed).
: "The lost correlation between heat shock protein 70 (HSPA1A) and plasminogen activator inhibitor-1 in patients with type 2 diabetes and albuminuria." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 361-5, (2016) (PubMed).
: "Disequilibrium of Blood Coagulation and Fibrinolytic System in Patients With Coronary Artery Ectasia." in: Medicine, Vol. 95, Issue 8, pp. e2779, (2016) (PubMed).
: "Suppression of plasminogen activator inhibitor-1 by inhaled nitric oxide attenuates the adverse effects of hyperoxia in a rat model of acute lung injury." in: Thrombosis research, Vol. 136, Issue 1, pp. 131-8, (2015) (PubMed).
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: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).
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- 抗原 See all PAI1 (SERPINE1) ELISA试剂盒
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
- 别名
- Plasminogen activator inhibitor-1 (PAI-1) (SERPINE1 产品)
- 别名
- PAI ELISA Kit, PAI-1 ELISA Kit, PAI1 ELISA Kit, PLANH1 ELISA Kit, SERPINE1 ELISA Kit, si:ch211-138a11.1 ELISA Kit, Planh1 ELISA Kit, PAI1A ELISA Kit, Pai1 ELISA Kit, Pai1aa ELISA Kit, Planh ELISA Kit, RATPAI1A ELISA Kit, serpin family E member 1 ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade E, member 1 ELISA Kit, SERPINE1 ELISA Kit, serpine1.L ELISA Kit, serpine1 ELISA Kit, Serpine1 ELISA Kit
- 背景
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Protein Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. .
Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator(tPA) and urokinase(uPA), the activators of plasminogen and hence fibrinolysis(the physiological breakdown of blood clots). It is a serine protease inhibitor(serpin) protein(SERPINE1). PAI-1 is mainly produced by the endothelium(cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome(7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis(a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states(such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.
Synonyms: Plasminogen activator inhibitor 1,PAI,PAI-1,Endothelial plasminogen activator inhibitor,Serpin E1,SERPINE1,PAI1, PLANH1,
Full Gene Name: Plasminogen activator inhibitor 1
Cellular Localisation: Secreted. - 基因ID
- 5054
- UniProt
- P05121
- 途径
- p53 Pathway, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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