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IL-10 ELISA 试剂盒

Name: IL-10 ELISA 试剂盒
SKU: ABIN411307
Availability: OutOfStock

This 小鼠 IL-10 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 IL-10 in Cell Culture Supernatant 和 Serum. This ELISA Kit has been cited in 76+ publications.

ELISA

1 image

(76 references)

产品编号 ABIN411307

发货至: 中国

Datasheet as PDF

Quick Overview for IL-10 ELISA 试剂盒 (ABIN411307)

抗原

See all IL-10 (IL10) ELISA试剂盒

IL-10 (IL10) (Interleukin 10 (IL10))

抗原表位

AA 19-178

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum

Discover our top product IL10 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-10

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: S19-S178

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<1pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: S19-S178
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IL10 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 10 (IL10) ELISA Kit
IL10 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

mouse IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-S178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-10 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-10 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 82, Standard deviation: 3.77, CV(%): 4.6
- Sample 2: n=16, Mean(pg/ml): 413, Standard deviation: 21.5, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 724, Standard deviation: 44.2, CV(%): 6.1,
- Sample 1: n=24, Mean(pg/ml): 113, Standard deviation: 7, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 367, Standard deviation: 24.96, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 628, Standard deviation: 46.5, CV(%): 7.4
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Liu, Xu, Chang, Qin, Yin, Yang: "4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-κB signaling in a ROS independent manner." in: Oncotarget, Vol. 7, Issue 27, pp. 40978-40991, (2018) (PubMed).

Lin, Chen, Xia, Yang: "MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3." in: Oncotarget, Vol. 7, Issue 31, pp. 48978-48994, (2018) (PubMed).

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Dong, Liu, Meng, Liu, Bi, Wu, Jin, Yao, Tang, Wang, Li, Zhang, Yu, Zhan, Chen, Ge, Yang, Li: "Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination." in: Scientific reports, Vol. 6, pp. 32710, (2018) (PubMed).

Shen, Zhang, Li, Liu, Yu, Hu, Hui, Guo, Zhang: "Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer." in: Oncotarget, Vol. 8, Issue 30, pp. 49395-49408, (2018) (PubMed).

Wang, Zhang, Yang, Zou, Wang, Liu, Liu, Zhang, Xiao, Wang: "Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1." in: International journal of molecular medicine, Vol. 40, Issue 2, pp. 427-437, (2018) (PubMed).

Dong, Han, Wang, Qin, Yang, Cao, Chen: "Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice." in: BMC gastroenterology, Vol. 17, Issue 1, pp. 82, (2018) (PubMed).

Zhang, Wu, Xu, He, Yang, Li, Li, Ao, Cheng, Jia: "The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia." in: Brain, behavior, and immunity, Vol. 66, pp. 332-346, (2018) (PubMed).

Wang, Dong, Wang, Liu, Sun, Li, Lin, Geng, Jin, Li: "Adolescent escitalopram prevents the effects of maternal separation on depression- and anxiety-like behaviours and regulates the levels of inflammatory cytokines in adult male mice." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 62, pp. 37-45, (2018) (PubMed).

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Lu, Liu, Jin, Chen, Liang, Qiu, Dai: "Herbal Components of a Novel Formula PSORI-CM02 Interdependently Suppress Allograft Rejection and Induce CD8+CD122+PD-1+ Regulatory T Cells." in: Frontiers in pharmacology, Vol. 9, pp. 88, (2018) (PubMed).

Wang, Liu, Wang, Yang, Li, Ding, Sun, Bai, Li: "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 2305683, (2018) (PubMed).

Zhang, Li, Wang, Xie, Li, Zuo, Kong, Wang, Wang: "Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis." in: Frontiers in microbiology, Vol. 9, pp. 909, (2018) (PubMed).

Wang, Cai, Li, Chen, Zhang, Li, Ding, Liu, Cui: "Detoxication mechanisms of Radix Tripterygium wilfordii via compatibility with Herba Lysimachia christinae in S180-bearing mice by involving Nrf2." in: Bioscience reports, Vol. 38, Issue 4, (2018) (PubMed).

Meng, Zhang, Wu, Wu, Li, Yan, Kopec-Harding, Wu: "In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580." in: International journal of chronic obstructive pulmonary disease, Vol. 11, pp. 909-17, (2017) (PubMed).

Chen, Wang, Zhang, Zheng, Hu: "In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly." in: Mediators of inflammation, Vol. 2016, pp. 3583684, (2017) (PubMed).

Wang, Xie, Yang, Zhang, Wang, Wu, Shen, Xie: "Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice." in: Scientific reports, Vol. 7, pp. 40402, (2017) (PubMed).

Mohi El-Din, Rashed, Mahmoud Haridy, Khalil, Mohamed Albadry: "Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice." in: Future science OA, Vol. 3, Issue 1, pp. FSO162, (2017) (PubMed).

Lin, Xia, Tu, Zhang, Zeng, Yang: "H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375." in: Frontiers in microbiology, Vol. 8, pp. 287, (2017) (PubMed).

Li, Fan, Xia, Xiao, Wang, Sun, Zhang, He: "The immunosuppressive characteristics of FB1 by inhibition of maturation and function of BMDCs." in: International immunopharmacology, Vol. 47, pp. 206-211, (2017) (PubMed).

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抗原 See all IL-10 (IL10) ELISA试剂盒

IL-10 (IL10) (Interleukin 10 (IL10))

别名

IL10

背景

Protein Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa.
Synonyms: Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,Il10,Il-10,
Full Gene Name: Interleukin-10
Cellular Localisation: Secreted.

基因ID

16153

UniProt

P18893

途径

Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints