Fibronectin 1 ELISA 试剂盒
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- 抗原 See all Fibronectin 1 (FN1) ELISA试剂盒
- Fibronectin 1 (FN1)
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Fibronectin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
- Expression system for standard: from plasma
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <15pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
- from plasma
- Featured
- Discover our best selling FN1 ELISA Kit
- Top Product
- Discover our top product FN1 ELISA Kit
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Fibronectin 1 (FN1) ELISA Kit
FN1 适用: 人 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fibronectin 1 (FN1) ELISA KitFN1 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fibronectin 1 (FN1) ELISA KitFN1 适用: 兔 Colorimetric Competition ELISA 1.25 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: Cow Colorimetric Sandwich ELISA 0.137-100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 兔 Colorimetric Sandwich ELISA 1.25-20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 12 fibronectin type-I domains.
Tissue Specificity: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
- 板类型
- Pre-coated
- 实验流程
- rat Fibronectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fibronectin has been precoated onto 96-well plates. Standards(from plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fibronectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Fibronectin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL rat Fibronectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat Fibronectin standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.19, Standard deviation: 0.044, CV(%): 3.7
- Sample 2: n=16, Mean(ng/ml): 2.25, Standard deviation: 0.117, CV(%): 5.2
- Sample 3: n=16, Mean(ng/ml): 5.13, Standard deviation: 0.231, CV(%): 4.5,
- Sample 1: n=24, Mean(ng/ml): 1.35, Standard deviation: 0.092, CV(%): 6.8
- Sample 2: n=24, Mean(ng/ml): 2.29, Standard deviation: 0.169, CV(%): 7.4
- Sample 3: n=24, Mean(ng/ml): 5.28, Standard deviation: 0.364, CV(%): 6.9
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Effect of Wujia Shenghua capsule on uterine bleeding following medically-induced incomplete abortion in rats during early pregnancy." in: Experimental and therapeutic medicine, Vol. 10, Issue 2, pp. 635-640, (2015) (PubMed).
: "Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway." in: International journal of endocrinology, Vol. 2014, pp. 658589, (2014) (PubMed).
: "Exendin-4 alleviates high glucose-induced rat mesangial cell dysfunction through the AMPK pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 33, Issue 2, pp. 423-32, (2014) (PubMed).
: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, (2013) (PubMed).
: "Cdc42-interacting protein-4 promotes TGF-?1-induced epithelial-mesenchymal transition and extracellular matrix deposition in renal proximal tubular epithelial cells." in: International journal of biological sciences, Vol. 8, Issue 6, pp. 859-69, (2012) (PubMed).
: "Comparison of mesenchymal stem cells released from poly(N-isopropylacrylamide) copolymer film and by trypsinization." in: Biomedical materials (Bristol, England), Vol. 7, Issue 3, pp. 35003, (2012) (PubMed).
: "Pigment epithelium-derived factor inhibits high glucose induced oxidative stress and fibrosis of cultured human glomerular mesangial cells." in: Saudi medical journal, Vol. 32, Issue 8, pp. 769-77, (2011) (PubMed).
: "Role of Sema4C in TGF-?1-induced mitogen-activated protein kinase activation and epithelial-mesenchymal transition in renal tubular epithelial cells." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 26, Issue 4, pp. 1149-56, (2011) (PubMed).
: "Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1." in: Biochimica et biophysica acta, Vol. 1800, Issue 3, pp. 380-4, (2010) (PubMed).
: "Inhibition of calcium-calmodulin-dependent kinase II suppresses cardiac fibroblast proliferation and extracellular matrix secretion." in: Journal of cardiovascular pharmacology, Vol. 55, Issue 1, pp. 96-105, (2010) (PubMed).
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: "Effect of Wujia Shenghua capsule on uterine bleeding following medically-induced incomplete abortion in rats during early pregnancy." in: Experimental and therapeutic medicine, Vol. 10, Issue 2, pp. 635-640, (2015) (PubMed).
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- 抗原 See all Fibronectin 1 (FN1) ELISA试剂盒
- Fibronectin 1 (FN1)
- 别名
- FN1 (FN1 产品)
- 别名
- FN1 ELISA Kit, FN ELISA Kit, cig ELISA Kit, fibronectin ELISA Kit, finc ELISA Kit, lets ELISA Kit, msf ELISA Kit, Fn ELISA Kit, fn2 ELISA Kit, fb80d10 ELISA Kit, wu:fb80d10 ELISA Kit, CIG ELISA Kit, ED-B ELISA Kit, FINC ELISA Kit, FNZ ELISA Kit, GFND ELISA Kit, GFND2 ELISA Kit, LETS ELISA Kit, MSF ELISA Kit, E330027I09 ELISA Kit, Fn-1 ELISA Kit, FIBNEC ELISA Kit, fn-1 ELISA Kit, fibronectin 1 ELISA Kit, fibronectin 1a ELISA Kit, fibronectin 1 S homeolog ELISA Kit, FN1 ELISA Kit, fn1 ELISA Kit, fn1a ELISA Kit, Fn1 ELISA Kit, fn1.S ELISA Kit
- 背景
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Protein Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). .
Background: Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from rat plasma with the molecular mass of 200-250KDa.
Synonyms: Fibronectin,FN,Anastellin,Fn1,
Full Gene Name: Fibronectin
Cellular Localisation: Secreted, extracellular space, extracellular matrix. - 基因ID
- 25661
- UniProt
- P04937
- 途径
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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