Fibronectin 1 ELISA 试剂盒
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北京 101111
Quick Overview for Fibronectin 1 ELISA 试剂盒 (ABIN411271)
抗原
See all Fibronectin 1 (FN1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human Fibronectin ELISA Kit to quantitate Human FN1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
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Analytical Method
- Quantitative
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特异性
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Target protein isolated from human plasma
Capture antibody and Detection antibody: polyclonal antibody from rabbit, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Fibronectin 1 (FN1) ELISA Kit
FN1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 人 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 小鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fibronectin 1 (FN1) ELISA KitFN1 适用: 大鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fibronectin 1 (FN1) ELISA KitFN1 适用: 兔 Colorimetric Competition ELISA 1.25 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 兔 Colorimetric Sandwich ELISA 1.25-20 ng/mL Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: Cow Colorimetric Sandwich ELISA 0.137-100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.6%, 5.7%, 5.7%
Inter-Assay Precision (CV%): 5.7%, 6.9%, 7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "In situ expansion of engineered human liver tissue in a mouse model of chronic liver disease." in: Science translational medicine, Vol. 9, Issue 399, (2018) (PubMed).
: "A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM)." in: PLoS ONE, Vol. 9, Issue 8, pp. e105365, (2014) (PubMed).
: "Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway." in: International journal of endocrinology, Vol. 2014, pp. 658589, (2014) (PubMed).
: "Exendin-4 alleviates high glucose-induced rat mesangial cell dysfunction through the AMPK pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 33, Issue 2, pp. 423-32, (2014) (PubMed).
: "Quantitative biocompatibility evaluation of nickel-free high-nitrogen stainless steel in vitro/in vivo." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 1, pp. 68-72, (2014) (PubMed).
: "IGF2BP1 promotes mesenchymal cell properties and migration of tumor-derived cells by enhancing the expression of LEF1 and SNAI2 (SLUG)." in: Nucleic acids research, Vol. 41, Issue 13, pp. 6618-36, (2013) (PubMed).
: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, (2013) (PubMed).
: "Promotion of initial cell adhesion on trisuccinimidyl citrate-modified nickel-free high-nitrogen stainless steel." in: Journal of materials science. Materials in medicine, Vol. 24, Issue 4, pp. 951-8, (2013) (PubMed).
: "Comparison of mesenchymal stem cells released from poly(N-isopropylacrylamide) copolymer film and by trypsinization." in: Biomedical materials (Bristol, England), Vol. 7, Issue 3, pp. 35003, (2012) (PubMed).
: "Cdc42-interacting protein-4 promotes TGF-?1-induced epithelial-mesenchymal transition and extracellular matrix deposition in renal proximal tubular epithelial cells." in: International journal of biological sciences, Vol. 8, Issue 6, pp. 859-69, (2012) (PubMed).
: "Pigment epithelium-derived factor inhibits high glucose induced oxidative stress and fibrosis of cultured human glomerular mesangial cells." in: Saudi medical journal, Vol. 32, Issue 8, pp. 769-77, (2011) (PubMed).
: "Role of Sema4C in TGF-?1-induced mitogen-activated protein kinase activation and epithelial-mesenchymal transition in renal tubular epithelial cells." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 26, Issue 4, pp. 1149-56, (2011) (PubMed).
: "Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1." in: Biochimica et biophysica acta, Vol. 1800, Issue 3, pp. 380-4, (2010) (PubMed).
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- Fibronectin 1 (FN1)
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别名
- FN1
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背景
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Background: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.
Gene Full Name: fibronectin 1
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基因ID
- 2335
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UniProt
- P02751
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途径
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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