EGFR ELISA 试剂盒
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北京 101111
Quick Overview for EGFR ELISA 试剂盒 (ABIN411266)
抗原
See all EGFR ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human soluble EGFR ELISA Kit to quantitate Human EGFR in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: M1-S645Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Epidermal Growth Factor Receptor (EGFR) ELISA Kit
EGFR 适用: 人 Colorimetric Sandwich ELISA 0.312-20 ng/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人, 小鼠, 大鼠 pTyr992 Colorimetric Cell ELISA Cell Culture Cells
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人, 小鼠, 大鼠 pTyr992 Colorimetric Cell ELISA Cell Culture Cells
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人 pTyr1068 Colorimetric Cell ELISA Cell Culture Cells
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人 pSer1070 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人 pSer1070 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人 pTyr992 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人 pTyr1086 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人 pTyr1068 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Epidermal Growth Factor Receptor (EGFR) ELISA KitEGFR 适用: 人 phosphorylated Colorimetric Cell ELISA Cell Culture Cells
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.7%, 5%, 7.4%
Inter-Assay Precision (CV%): 6%, 5.9%, 7.5% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "The inhibitory effect of a new scFv/tP protein as siRNA delivery system to target hWAPL in cervical carcinoma." in: Molecular and cellular biochemistry, Vol. 391, Issue 1-2, pp. 77-84, (2014) (PubMed).
: "Predicting cetuximab accumulation in KRAS wild-type and KRAS mutant colorectal cancer using 64Cu-labeled cetuximab positron emission tomography." in: Cancer science, Vol. 103, Issue 3, pp. 600-5, (2012) (PubMed).
: "Expression of midkine and endoglin in breast carcinomas with different immunohistochemical profiles." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 119, Issue 2, pp. 103-10, (2011) (PubMed).
: "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1." in: Biology of reproduction, Vol. 80, Issue 3, pp. 528-36, (2009) (PubMed).
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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别名
- EGFR
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背景
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Background: The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
Gene Full Name: epidermal growth factor receptor
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基因ID
- 1956
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UniProt
- P00533
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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