EGF ELISA 试剂盒
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Quick Overview for EGF ELISA 试剂盒 (ABIN3198526)
抗原
See all EGF ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 7.81 pg/mL
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
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Analytical Method
- Quantitative
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特异性
- EGF ELISA Kit, Human
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Epidermal Growth Factor (EGF) ELISA Kit
EGF 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 人 Colorimetric Sandwich ELISA 3.906 pg/mL - 250 pg/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 人 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 小鸡 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 大鼠 Colorimetric Sandwich ELISA 3.906 pg/mL - 250 pg/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- EGF (Epidermal Growth Factor (EGF))
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别名
- EGF
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背景
- Epidermal growth factor (EGF) is the founding member of the EGF-family including HB-EGF, TGF-α, amphiregulin, epiregulin, epigen, betacellulin, NGR1, NGR2, NGR3 and NGR4. EGF can be found in macrophages, urine, saliva, milk, and plasma, is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF and/or EGFR have been exploited to develop imaging methods and targeted therapies against cancers overexpressing EGFR.
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基因ID
- 1950
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NCBI登录号
- NM_001178130
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UniProt
- P01133
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原 See all EGF ELISA试剂盒
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