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EGFR ELISA 试剂盒

EGFR 适用: 小鼠 AA 25-647 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN3044705
发货至: 中国
  • 抗原 See all EGFR ELISA试剂盒
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    抗原表位
    AA 25-647
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156-10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse EGFR
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: L25-S647
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <15pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Immunogen sequence: L25-S647
    Featured
    Discover our best selling EGFR ELISA Kit
    Top Product
    Discover our top product EGFR ELISA Kit
  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    板类型
    Pre-coated
    实验流程
    mouse EGFR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EGFR has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L25-S647) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGFR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EGFR amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse EGFR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse EGFR standard solution and each sample be measured in duplicate.

    限制
    仅限研究用
  • 缓冲液
    heparin or EDTA
    注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all EGFR ELISA试剂盒
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    别名
    EGFR (EGFR 产品)
    别名
    C-erb ELISA Kit, CG10079 ELISA Kit, D-EGFR ELISA Kit, D-Egf ELISA Kit, DEGFR ELISA Kit, DER ELISA Kit, DER flb ELISA Kit, DER/EGFR ELISA Kit, DER/faint little ball ELISA Kit, DER/top ELISA Kit, DER/torpedo ELISA Kit, DER1 ELISA Kit, DEgfr ELISA Kit, Degfr ELISA Kit, Der ELISA Kit, DmHD-33 ELISA Kit, Dmel\\CG10079 ELISA Kit, EFG-R ELISA Kit, EGF-R ELISA Kit, EGFR ELISA Kit, EGFr ELISA Kit, EGfr ELISA Kit, EK2-6 ELISA Kit, Egf ELISA Kit, Egf-r ELISA Kit, EgfR ELISA Kit, El ELISA Kit, Elp ELISA Kit, Elp-1 ELISA Kit, Elp-B1 ELISA Kit, Elp-B1RB1 ELISA Kit, HD-33 ELISA Kit, TOP ELISA Kit, Torpedo/DER ELISA Kit, Torpedo/Egfr ELISA Kit, c-erbB ELISA Kit, d-egf-r ELISA Kit, dEGFR ELISA Kit, dEGFR1 ELISA Kit, dEgfr ELISA Kit, der ELISA Kit, egfr ELISA Kit, flb ELISA Kit, l(2)05351 ELISA Kit, l(2)09261 ELISA Kit, l(2)57DEFa ELISA Kit, l(2)57EFa ELISA Kit, l(2)57Ea ELISA Kit, mor1 ELISA Kit, top ELISA Kit, top/DER ELISA Kit, top/flb ELISA Kit, torpedo/Egfr ELISA Kit, torpedo/egfr ELISA Kit, EGFR12 ELISA Kit, EGFR15 ELISA Kit, egfr1 ELISA Kit, Erbb2 ELISA Kit, ERBB ELISA Kit, ERBB1 ELISA Kit, HER1 ELISA Kit, PIG61 ELISA Kit, mENA ELISA Kit, ErbB-1 ELISA Kit, Errp ELISA Kit, 9030024J15Rik ELISA Kit, AI552599 ELISA Kit, Erbb ELISA Kit, Errb1 ELISA Kit, Wa5 ELISA Kit, wa-2 ELISA Kit, wa2 ELISA Kit, epidermal growth factor receptor ELISA Kit, Epidermal growth factor receptor ELISA Kit, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) ELISA Kit, EGFR ELISA Kit, Egfr ELISA Kit, egfra ELISA Kit, egfr1 ELISA Kit, LOC5564544 ELISA Kit
    背景

    Protein Function: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. .

    Background: The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor i± (TGFi±). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

    Synonyms: Epidermal growth factor receptor,2.7.10.1,Egfr,

    Full Gene Name: Epidermal growth factor receptor

    Cellular Localisation: Cell membrane, Single-pass type I membrane protein. Endoplasmic reticulum membrane, Single-pass type I membrane protein . Golgi apparatus membrane, Single-pass type I membrane protein . Nucleus membrane, Single-pass type I membrane protein . Endosome. Endosome membrane. Nucleus . In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (By similarity)..
    基因ID
    13649
    UniProt
    Q01279
    途径
    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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