Caspase 8 ELISA 试剂盒
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Quick Overview for Caspase 8 ELISA 试剂盒 (ABIN3044700)
抗原
See all Caspase 8 (CASP8) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Human Caspase 8/CASP8 ELISA Kit to quantitate Human CASP8 in cell culture supernatantsand serum.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: S217-D384 & L385-D479Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Caspase 8 (CASP8) ELISA Kit
CASP8 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: 犬 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: 小鸡 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: 人 cleaved Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.4%, 6.4%, 5.4%
Inter-Assay Precision (CV%): 7.3%, 8.2%, 5.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Caspase 8 (CASP8)
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别名
- CASP8
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背景
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Background: Caspase-8 is a caspase protein, encoded by the CASP8 gene. It is mapped to human chromosome 2q33-q34 and mouse chromosome 1B-proximal C. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. And this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Gene Full Name: caspase 8
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基因ID
- 841
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UniProt
- Q14790
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途径
- Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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