PIK3CA ELISA 试剂盒
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Quick Overview for PIK3CA ELISA 试剂盒 (ABIN2950303)
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See all PIK3CA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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Analytical Method
- Quantitative
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灵敏度
- < 0.054 ng/mL
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组件
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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试剂未包括
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) ELISA Kit
PIK3CA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) ELISA KitPIK3CA 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) ELISA KitPIK3CA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) ELISA KitPIK3CA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) ELISA KitPIK3CA 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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样本量
- 100 µL
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板类型
- Pre-coated
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试剂准备
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
- 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
- 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
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实验流程
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Set standard, test samples and control wells.
- 2) Aliquot 100 μL of diluted standard into the standard wells.
- 3) Aliquot 100 μL of Standard Diluent buffer into control (zero) well.
- 4) Aliquot 100 μL of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.
- 5) Aliquot 100 μL of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
- 6) Wash 3 times.
- 7) Aliquot 100 μL of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
- 8) Wash 5 times.
- 9) Aliquot 90 μL of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
- 10) Aliquot 50 μL of Stop Solution.
- 11) Measure the OD at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Shipped at 4°C. Upon receipt, store the kit according to the storage instruction in the kit's manual.The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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有效期
- 6 months
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- PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))
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别名
- Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform
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途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling
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