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IGFBP5 ELISA 试剂盒

Pig IGFBP5 ELISA Kit, Colorimetric is an assay for quantification of Pig IGFBP5.

产品编号 ABIN2748200

发货至: 中国

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中国
北京 101111

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Beijing Economic Technological Development Area

Room 801-803

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Quick Overview for IGFBP5 ELISA 试剂盒 (ABIN2748200)

抗原

See all IGFBP5 ELISA试剂盒

IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

适用

Pig

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.13 ng/mL - 40 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product IGFBP5 ELISA Kit

最低检测浓度

0.13 ng/mL

原理

Porcine IGFBP-5 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytical Method

Quantitative

特异性

This ELISA antibody pair detects porcine IGFBP-5. Other species not determined.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
  - Distilled or deionized water
  - Precision pipettes to deliver 2 μl to 1 μl volumes
  - Adjustable 1-25 μl pipettes for reagent preparation
  - 100 μl and 1 liter graduated cylinders
  - Tubes to prepare standard and sample dilutions
  - Absorbent paper
  - Microplate reader capable of measuring absorbance at 450nm
  - Log-log graph paper or computer and software for ELISA data analysis
  ,
  - Pre-Coated 96-well Strip Microplate
  - Wash Buffer
  - Stop Solution
  - Assay Diluent(s)
  - Lyophilized Standard
  - Biotinylated Detection Antibody
  - Streptavidin-Conjugated HRP
  - TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
    Distilled or deionized water
    Precision pipettes to deliver 2 μl to 1 μl volumes
    Adjustable 1-25 μl pipettes for reagent preparation
    100 μl and 1 liter graduated cylinders
    Tubes to prepare standard and sample dilutions
    Absorbent paper
    Microplate reader capable of measuring absorbance at 450nm
    Log-log graph paper or computer and software for ELISA data analysis
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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IGFBP5 适用: 小鼠 AA 20-271 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

应用备注

Suggested Plasma Dilution: 2 fold

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
实验流程

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

限制

仅限研究用
储存条件

-20 °C

储存方法

May be stored for up to 6 months at 2^° to 8^°C from the date of shipment. Standard (recombinant protein) should be stored at -20^°C or -80^°C (recommended at -80^°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2^° to 8^°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20^°C.

有效期

6 months
抗原 See all IGFBP5 ELISA试剂盒

IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

别名

IGFBP-5

背景

Gene Symbols: IGFBP5
Protein Name (Synonyms): Insulin-like growth factor-binding protein 5 (IBP-5) (IGF-binding protein 5) (IGFBP-5)

基因ID

397182

UniProt

Q28985

途径

WNT signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding