GDNF ELISA 试剂盒
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- 抗原 See all GDNF ELISA试剂盒
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 60-15.000 pg/mL
- 最低检测浓度
- 60 pg/mL
- 应用范围
- ELISA
- 原理
- Rat GDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects rat and human GDNF. Other species not determined.
- 灵敏度
- 60 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling GDNF ELISA Kit
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- Discover our top product GDNF ELISA Kit
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Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitGDNF 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitGDNF 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitGDNF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitSmall Sample GDNF 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitGDNF 适用: 小鼠 AA 78-211 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitGDNF 适用: 人 AA 109-211 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitGDNF 适用: 大鼠 AA 78-211 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitGDNF 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA KitGDNF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all GDNF ELISA试剂盒
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
- 别名
- GDNF (GDNF 产品)
- 别名
- gdnf ELISA Kit, GDNF ELISA Kit, AI385739 ELISA Kit, gdnfa ELISA Kit, hfb1-gdnf ELISA Kit, ATF1 ELISA Kit, ATF2 ELISA Kit, HFB1-GDNF ELISA Kit, HSCR3 ELISA Kit, glial cell derived neurotrophic factor ELISA Kit, glial cell derived neurotrophic factor a ELISA Kit, glial cell line derived neurotrophic factor ELISA Kit, glial cell derived neurotrophic factor L homeolog ELISA Kit, Gdnf ELISA Kit, gdnfa ELISA Kit, GDNF ELISA Kit, gdnf.L ELISA Kit
- 背景
- Glial cell line-derived neurotrophic factor (Astrocyte-derived trophic factor) (ATF)
- 基因ID
- 25453
- UniProt
- Q07731
- 途径
- RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
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