VEGFR2/CD309 ELISA 试剂盒
VEGFR2
适用: 小鼠
Colorimetric
Sandwich ELISA
80-20.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒
- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 80-20.000 pg/mL
- 最低检测浓度
- 80 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse VEGFR2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30L, CD30, CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1.
- 灵敏度
- 80 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒
- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
- 别名
- VEGFR2 (VEGFR2 产品)
- 别名
- CD309 ELISA Kit, FLK1 ELISA Kit, VEGFR ELISA Kit, VEGFR2 ELISA Kit, 6130401C07 ELISA Kit, Flk-1 ELISA Kit, Flk1 ELISA Kit, Krd-1 ELISA Kit, Ly73 ELISA Kit, VEGFR-2 ELISA Kit, sVEGFR-2 ELISA Kit, Vegfr-2 ELISA Kit, FLK-1 ELISA Kit, flk-1 ELISA Kit, vegfr-2 ELISA Kit, KDR ELISA Kit, FLK/KDR ELISA Kit, flk1 ELISA Kit, flk1b ELISA Kit, kdrb ELISA Kit, si:busm1-205d10.1 ELISA Kit, si:ch211-254j6.1 ELISA Kit, si:ch211-278f21.4 ELISA Kit, wu:fc31a09 ELISA Kit, fk52c05 ELISA Kit, flk ELISA Kit, kdr ELISA Kit, kdra ELISA Kit, vegfr2 ELISA Kit, vegfr4 ELISA Kit, vegr2 ELISA Kit, wu:fk52c05 ELISA Kit, kinase insert domain receptor ELISA Kit, kinase insert domain protein receptor ELISA Kit, vascular endothelial growth factor receptor kdr-like ELISA Kit, kinase insert domain receptor (a type III receptor tyrosine kinase) ELISA Kit, kinase insert domain receptor like ELISA Kit, KDR ELISA Kit, Kdr ELISA Kit, LOC100136647 ELISA Kit, kdr ELISA Kit, kdrl ELISA Kit
- 背景
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Gene Names: Kdr
Protein names: Putative uncharacterized protein (Fragment)
- UniProt
- Q3UQZ6
- 途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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