IGFBP5 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for IGFBP5 ELISA 试剂盒 (ABIN2703127)
抗原
See all IGFBP5 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 25 pg/mL
-
原理
- Rat IGFBP-5 ELISA Kit for cell culture supernatants, plasma, and serum samples.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair detects rat IGFBP-5 and mouse IGFBP-5.
-
灵敏度
- 25 pg/mL
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit
IGFBP5 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 AA 20-271 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 小鼠 AA 20-271 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 犬 Colorimetric Competition ELISA 2.5-50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Recommended Dilution for serum and plasma samples100 fold
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
有效期
- 6 months
-
-
- IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
-
别名
- IGFBP-5
-
背景
-
Gene Names: Igfbp5 Igfbp-5
Protein names: Insulin-like growth factor-binding protein 5 (IBP-5) (IGF-binding protein 5) (IGFBP-5)
-
基因ID
- 25285
-
UniProt
- P24594
-
途径
- WNT signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
抗原 See all IGFBP5 ELISA试剂盒
-
