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Galectin 3 ELISA 试剂盒

人 Galectin 3 ELISA Kit, Colorimetric is an assay for quantification of 人 Galectin 3, that has been mentioned in 1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN2703038

发货至: 中国

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Quick Overview for Galectin 3 ELISA 试剂盒 (ABIN2703038)

抗原

See all Galectin 3 (LGALS3) ELISA试剂盒

Galectin 3 (LGALS3)

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.6-200 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product LGALS3 ELISA Kit

最低检测浓度

0.6 ng/mL

原理

Human Galectin-3 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method

Quantitative

特异性

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM-9, Angiopoietin 2, APRIL, BMP-2, BMP-9, C5a, Cathepsin L, CD200, CD97, Chemerin, DcR3, FABP2, FAP, FGF-19, HGF R, IFN-gamma, IGF-2, IGF-2 R, IL-1R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PD-1, PGRP-5, Serpin A4, sFRP-3, Thrombomodulin, TLR2, TRAIL R1, Transferrin,WIF-1

灵敏度

0.6 ng/mL

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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LGALS3 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Galectin 3 (LGALS3) ELISA Kit
Small Sample LGALS3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Galectin 3 (LGALS3) ELISA Kit
LGALS3 适用: 人 Colorimetric Sandwich ELISA 0.625-40 ng/mL Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenate

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LGALS3 适用: 人 AA 2-250 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

应用备注

Recommended Dilution for serum and plasma samples2 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
Nirala, Perumal, Gohil: "Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction." in: Diabetes & vascular disease research, Vol. 12, Issue 4, pp. 290-7, (2015) (PubMed).
抗原 See all Galectin 3 (LGALS3) ELISA试剂盒

Galectin 3 (LGALS3)

别名

Galectin-3

物质类

Chemical

背景

Gene Names: LGALS3 MAC2
Protein names: Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (Galactoside-binding protein) (GALBP) (IgE-binding protein) (L-31) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen)

基因ID

3958

UniProt

P17931

途径

RTK signaling