Galectin 3 ELISA 试剂盒
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- 抗原 See all Galectin 3 (LGALS3) ELISA试剂盒
- Galectin 3 (LGALS3)
- 抗原表位
- AA 2-250
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-3/LGALS3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: A2-I250 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: A2-I250 - Featured
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Galectin 3 (LGALS3) ELISA Kit
LGALS3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 3 (LGALS3) ELISA KitLGALS3 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenate
Galectin 3 (LGALS3) ELISA KitLGALS3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 3 (LGALS3) ELISA KitLGALS3 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Galectin 3 (LGALS3) ELISA KitLGALS3 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Galectin 3 (LGALS3) ELISA KitLGALS3 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 3 (LGALS3) ELISA KitSmall Sample LGALS3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 3 (LGALS3) ELISA KitLGALS3 适用: 小鼠 AA 2-264 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Galectin 3 (LGALS3) ELISA KitLGALS3 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Galectin 3 (LGALS3) ELISA KitLGALS3 适用: 犬 Colorimetric Sandwich ELISA 2-500 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 galectin domain.
Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .
- 板类型
- Pre-coated
- 实验流程
- human Galectin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-3 has been precoated onto 96-well plates. Standards(E.coli, A2-I250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-3 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Galectin-3 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 624, Standard deviation: 27.46, CV(%): 4.4
- Sample 2: n=16, Mean(pg/ml): 3138, Standard deviation: 163.2, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 6618, Standard deviation: 271.3, CV(%): 4.1,
- Sample 1: n=24, Mean(pg/ml): 615, Standard deviation: 54.12, CV(%): 8.8
- Sample 2: n=24, Mean(pg/ml): 3449, Standard deviation: 279.4, CV(%): 8.1
- Sample 3: n=24, Mean(pg/ml): 6296, Standard deviation: 453.3, CV(%): 7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Galectin 3 (LGALS3) ELISA试剂盒
- Galectin 3 (LGALS3)
- 别名
- LGALS3 (LGALS3 产品)
- 别名
- CBP 35 ELISA Kit, Gal-3 ELISA Kit, Galectin-3 ELISA Kit, leg3 ELISA Kit, galactoside-binding ELISA Kit, galectin-3 ELISA Kit, lectin ELISA Kit, soluble ELISA Kit, CBP35 ELISA Kit, GAL3 ELISA Kit, GALBP ELISA Kit, GALIG ELISA Kit, L31 ELISA Kit, LGALS2 ELISA Kit, MAC2 ELISA Kit, MGC52947 ELISA Kit, MGC75803 ELISA Kit, LGALS3 ELISA Kit, gal-3 ELISA Kit, GBP ELISA Kit, L-34 ELISA Kit, Mac-2 ELISA Kit, gal3 ELISA Kit, galectin 3 ELISA Kit, Galectin-3 ELISA Kit, avian beta-defensin 3 ELISA Kit, lectin, galactoside-binding, soluble, 3 L homeolog ELISA Kit, lectin, galactoside-binding, soluble, 3 ELISA Kit, galectin 2 ELISA Kit, lectin, galactose binding, soluble 3 ELISA Kit, LGALS3 ELISA Kit, leg3 ELISA Kit, AvBD3 ELISA Kit, lgals3.L ELISA Kit, lgals3 ELISA Kit, LGALS2 ELISA Kit, Lgals3 ELISA Kit
- 物质类
- Chemical
- 背景
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Protein Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. .
Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21â€"q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
Synonyms: Galectin-3,Gal-3,35 kDa lectin,Carbohydrate-binding protein 35,CBP 35,Galactose-specific lectin 3,Galactoside-binding protein,GALBP,IgE-binding protein,L-31,Laminin-binding protein,Lectin L-29,Mac-2 antigen,LGALS3,MAC2,
Full Gene Name: Galectin-3
Cellular Localisation: Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface. - 基因ID
- 3958
- UniProt
- P17931
- 途径
- RTK signaling
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