CCL2 ELISA 试剂盒
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Quick Overview for CCL2 ELISA 试剂盒 (ABIN2690571)
抗原
See all CCL2 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- The Human MCP-1 ELISA Kit is for the quantitative determination of human MCP-1 in serum, plasma, and cell culture supernatant.
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Analytical Method
- Quantitative
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组件
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Antibody Coated Wells - 2 plates of 96 breakable wells (12 strips x 8 wells per plate)
Detection Antibody - 30 mL
Standards - 4 vials, lyophilized
Enzyme Concentrate (250x) - 150 μL
Standard/Sample Diluent - 30 mL
ELISA Diluent - 12 mL
Wash Concentrate (20x) - 100 mL
TMB One-Step Substrate Reagent - 30 mL
Stop Solution - 13 mL
Plate Sealers - 4 each
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Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 小鼠, 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 人 AA 24-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 猴 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 小鼠 AA 24-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 大鼠 AA 24-148 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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BD OptEIA™ Human MCP-1 ELISA Kit
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
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1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).
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: "Chemokines." in: Blood, Vol. 90, Issue 3, pp. 909-28, (1997) (PubMed).
: "The human homolog of the JE gene encodes a monocyte secretory protein." in: Molecular and cellular biology, Vol. 9, Issue 11, pp. 4687-95, (1990) (PubMed).
: "A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes." in: Journal of immunological methods, Vol. 120, Issue 1, pp. 125-31, (1989) (PubMed).
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: "Chemokines." in: Blood, Vol. 90, Issue 3, pp. 909-28, (1997) (PubMed).
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- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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别名
- MCP-1
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背景
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Human monocyte chemoattractant protein-1 (MCP-1), previously known as MCAF (monocyte chemotactic and activating factor), is a member of the CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原 See all CCL2 ELISA试剂盒
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