EGF ELISA 试剂盒
Quick Overview for EGF ELISA 试剂盒 (ABIN2648643)
抗原
See all EGF ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Human Epidermal Growth Factor (EGF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Epidermal Growth Factor (EGF) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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产品特性
- Human Epidermal Growth Factor (EGF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Epidermal Growth Factor (EGF) ELISA Kit
EGF 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 人 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Epidermal Growth Factor (EGF) ELISA KitWide Range EGF 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 小鸡 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 大鼠 AA 974-1026 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
Epidermal Growth Factor (EGF) ELISA KitEGF 适用: 人 AA 971-1023 Colorimetric Sandwich ELISA 4.7-300 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验时间
- 3.5 h
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板类型
- Pre-coated
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实验流程
- Human Epidermal Growth Factor (EGF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for EGF is added to the wells and binds to the combination of capture antibody- EGF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of EGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven EGF standard dilutions and EGF sample concentration determined.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- EGF (Epidermal Growth Factor (EGF))
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别名
- EGF
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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