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CCL2 ELISA 试剂盒

Name: CCL2 ELISA 试剂盒
SKU: ABIN2112006
Availability: OutOfStock

This 小鼠 CCL2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CCL2.

产品编号 ABIN2112006

发货至: 中国

Quick Overview for CCL2 ELISA 试剂盒 (ABIN2112006)

抗原

See all CCL2 ELISA试剂盒

CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

适用

小鼠

检测方法

Colorimetric

实验类型

Competition ELISA

检测范围

7.8-500 pg/mL

应用范围

ELISA

Discover our top product CCL2 ELISA Kit

最低检测浓度

7.8 pg/mL

品牌

BioAssay™

灵敏度

< 1.7 pg/mL

组件

MCP-1 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MCP-1 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION
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板类型

Pre-coated

实验流程

Elevated MCP-1 levels are associated with autoimmune disease, allergic inflammation, atherosclerosis, glomerulonephritis, granuloma formation. This ELISA kit provides a tool for studying MCP-1 expression and its relationship with various diseases in animal model.This mouse MCP-1 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse MCP-1. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse MCP-1, if present, will bind and become immobilized by the antibody pre-coated on the wells. A biotin conjugated antibody specific for mouse MCP-1 is added to each well. The biotin conjugated antibody will bind to the mouse MCP-1 on the plate. The microtiter plate wells are thoroughly washed to remove unbound biotin conjugate and other components of the sample. Avidin has a very high affinity to biotin. In order to quantify the amount of mouse MCP-1 present in the sample, a standardized preparation of avidin conjugated horseradish peroxidase (HRP) is added to each well. Avidin-HRPwill bind to the biotin on plate during incubation. The wells are thoroughly washed to remove all unbound Avidin-HRPconjugate and aTMB(3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only wells to which mouse MCP-1, Biotin conjugate and Avidin-HRPare attached will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

限制

仅限研究用
储存条件

4 °C/-20 °C
抗原 See all CCL2 ELISA试剂盒

CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

别名

MCP-1

途径

Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection