WIF1 ELISA 试剂盒
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Quick Overview for WIF1 ELISA 试剂盒 (ABIN2044847)
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See all WIF1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 15.62 pg/mL
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灵敏度
- < 6.9 pg/mL
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WNT Inhibitory Factor 1 (WIF1) ELISA Kit
WIF1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 小鼠 AA 29-379 Colorimetric Sandwich ELISA
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 人 AA 29-379 Colorimetric Sandwich ELISA
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 大鼠 AA 29-379 Colorimetric Sandwich ELISA
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 人 Colorimetric Sandwich ELISA 0.1-25 ng/mL Cell Culture Supernatant, Plasma, Serum
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板类型
- Pre-coated
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实验流程
- WNT Inhibitory Factor 1 (WIF1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT Inhibitory Factor 1 (WIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WNT Inhibitory Factor 1 (WIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- WIF1 (WNT Inhibitory Factor 1 (WIF1))
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途径
- WNT signaling, Positive Regulation of fat Cell Differentiation
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