WIF1 ELISA 试剂盒
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Quick Overview for WIF1 ELISA 试剂盒 (ABIN5510690)
抗原
See all WIF1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 23.4 pg/mL
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原理
- Sandwich Mouse WIF1 ELISA Kit to quantitate Mouse Wif1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: G29-W379Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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WNT Inhibitory Factor 1 (WIF1) ELISA Kit
WIF1 适用: 人 Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 人 AA 29-379 Colorimetric Sandwich ELISA 23.4 pg/mL - 1500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 大鼠 AA 29-379 Colorimetric Sandwich ELISA 23.4 pg/mL - 1500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 人 Colorimetric Sandwich ELISA 0.1-25 ng/mL Cell Culture Supernatant, Plasma, Serum
WNT Inhibitory Factor 1 (WIF1) ELISA KitWIF1 适用: 人 Colorimetric Sandwich ELISA 47-3000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.8%, 7.7%, 4%
Inter-Assay Precision (CV%): 4.8%, 9.3%, 4.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- WIF1 (WNT Inhibitory Factor 1 (WIF1))
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别名
- Wif1
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背景
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Background: Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Gene Full Name: Wnt inhibitory factor 1
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基因ID
- 24117
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UniProt
- Q9WUA1
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途径
- WNT signaling, Positive Regulation of fat Cell Differentiation
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