VEGFR2/CD309 ELISA 试剂盒
Quick Overview for VEGFR2/CD309 ELISA 试剂盒 (ABIN2044781)
抗原
See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 1.56 ng/mL
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灵敏度
- < 0.62 ng/mL
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VEGF Receptor 2 (VEGFR2) ELISA Kit
VEGFR2 适用: 人 AA 20-764 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 小鼠 AA 20-762 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 人 Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Plasma, Serum
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板类型
- Pre-coated
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实验流程
- Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor Receptor 2 (VEGFR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor Receptor 2 (VEGFR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
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别名
- Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
抗原 See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒
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