MAPK12 ELISA 试剂盒
This 人 MAPK12 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 MAPK12.
Quick Overview for MAPK12 ELISA 试剂盒 (ABIN2043983)
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See all MAPK12 ELISA试剂盒
MAPK12
(Mitogen-Activated Protein Kinase 12 (MAPK12))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.072 ng/mL
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Mitogen-Activated Protein Kinase 12 (MAPK12) ELISA Kit
MAPK12 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Mitogen Activated Protein Kinase 12 (MAPK12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase 12 (MAPK12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mitogen Activated Protein Kinase 12 (MAPK12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))
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别名
- Mitogen Activated Protein Kinase 12 (MAPK12)
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins
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