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MAPK12 ELISA 试剂盒

MAPK12 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690925
发货至: 中国
  • 抗原 See all MAPK12 ELISA试剂盒
    MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human p38 gamma/MAPK12/ERK-6 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human p38 gamma.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all MAPK12 ELISA试剂盒
    MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))
    别名
    p38 gamma (MAPK12/ERK-6) (MAPK12 产品)
    别名
    ATMPK12 ELISA Kit, MAPK12 ELISA Kit, T3F17.28 ELISA Kit, mitogen-activated protein kinase 12 ELISA Kit, MGC160082 ELISA Kit, si:dkey-14d8.5 ELISA Kit, erk6 ELISA Kit, etID309866.18 ELISA Kit, mapk12 ELISA Kit, sapk3 ELISA Kit, wu:fa05c12 ELISA Kit, zgc:101695 ELISA Kit, ERK3 ELISA Kit, ERK6 ELISA Kit, P38GAMMA ELISA Kit, PRKM12 ELISA Kit, SAPK-3 ELISA Kit, SAPK3 ELISA Kit, AW123708 ELISA Kit, Erk6 ELISA Kit, P38gamma ELISA Kit, Prkm12 ELISA Kit, Sapk3 ELISA Kit, mitogen-activated protein kinase 12 ELISA Kit, mitogen-activated protein kinase 12b ELISA Kit, mitogen-activated protein kinase 12a ELISA Kit, MPK12 ELISA Kit, MAPK12 ELISA Kit, mapk12b ELISA Kit, mapk12a ELISA Kit, Mapk12 ELISA Kit
    基因ID
    6300
    UniProt
    P53778
    途径
    MAPK Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins
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