HLA-B ELISA 试剂盒
This 人 HLA-B ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 HLA-B.
Quick Overview for HLA-B ELISA 试剂盒 (ABIN2043915)
抗原
See all HLA-B products
HLA-B
(Major Histocompatibility Complex, Class I, B (HLA-B))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
1.56-100 ng/mL
应用范围
ELISA
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最低检测浓度
- 1.56 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.63 ng/mL
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Major Histocompatibility Complex, Class I, B (HLA-B) ELISA Kit
HLA-B 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Major Histocompatibility Complex Class I B (MHCB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I B (MHCB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I B (MHCB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I B (MHCB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class I B (MHCB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))
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别名
- Major Histocompatibility Complex Class I B (MHCB)
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原 See all HLA-B products
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