HLAG ELISA 试剂盒
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Quick Overview for HLAG ELISA 试剂盒 (ABIN2043001)
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See all HLAG ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.136 ng/mL
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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) ELISA Kit
HLAG 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) ELISA KitHLAG 适用: 人 Colorimetric Sandwich ELISA 0.38 ng/mL - 24 ng/mL Plasma, Serum, Tissue Homogenate
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) ELISA KitHLAG 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) ELISA KitHLAG 适用: 人 Colorimetric Sandwich ELISA 1.25-80 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Beta-2-Microglobulin (b2M) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin (b2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-2-Microglobulin (b2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin (b2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Beta-2-Microglobulin (b2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
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别名
- b2-Microglobulin (b2M)
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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