PAI1 ELISA 试剂盒
Quick Overview for PAI1 ELISA 试剂盒 (ABIN2042630)
抗原
See all PAI1 (SERPINE1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
-
-
最低检测浓度
- 6 pg/mL
-
交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
96-well plate pre-coated with anti-PAI-1, Human
Lyophilized recombinant PAI-1, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
-
-
-
-
板类型
- Pre-coated
-
实验流程
- Sandwich ELISA quantitative detection kit for Human PAI-1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43kD.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C/-20 °C
-
-
- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
-
别名
- PAI-1
-
基因ID
- 5054
-
UniProt
- P05121
-
途径
- p53 Pathway, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
抗原 See all PAI1 (SERPINE1) ELISA试剂盒
-