NGFB ELISA 试剂盒
Quick Overview for NGFB ELISA 试剂盒 (ABIN2042620)
抗原
See all NGFB ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.2 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
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灵敏度
- < 1 pg/mL
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组件
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96-well plate pre-coated with anti-NGF/NGF beta, Mouse
Lyophilized recombinant NGF/NGF beta, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse NGF/NGF beta in cell culture supernates and serum.Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- NGFB (Nerve Growth Factor beta (NGFB))
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基因ID
- 18049
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UniProt
- P01139
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途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
抗原 See all NGFB ELISA试剂盒
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