NGFB ELISA 试剂盒
Quick Overview for NGFB ELISA 试剂盒 (ABIN2042619)
抗原
See all NGFB ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 15.6 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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A: Microtiter Strip, 1x96 wells
B: NGF Standard, 2x10 ng
C: Anti-human NGF (Biotin), 1x130 µL
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板类型
- Pre-coated
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实验流程
- NGF/NGF beta BioAssay-ELISA Kit, Human is a sandwich ELISA quantitative detection kit for human NGF/NGF beta in cell culture supernates and serum.Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF (1). NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis (2). The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- NGFB (Nerve Growth Factor beta (NGFB))
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基因ID
- 4803
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UniProt
- P01138
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途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
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