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IL12 ELISA 试剂盒

This 小鼠 IL12 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 IL12.

产品编号 ABIN2042522

发货至: 中国

Quick Overview for IL12 ELISA 试剂盒 (ABIN2042522)

抗原

See all IL12 ELISA试剂盒

IL12 (Interleukin 12 (IL12))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA

Discover our top product IL12 ELISA Kit

最低检测浓度

15.6 pg/mL

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 1.5 pg/mL

组件

96-well plate pre-coated with anti-IL-12(p70), Mouse
Lyophilized recombinant IL-12(p70), Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Interleukin 12 (IL12) ELISA Kit
IL12 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 12 (IL12) ELISA Kit
IL12 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

板类型

Pre-coated

实验流程

Sandwich ELISA quantitative detection kit for Mouse IL-12(p70) in cell culture supernates and serum.Interleukin (IL)-12 is a 70kD cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

限制

仅限研究用
储存条件

4 °C/-20 °C
抗原 See all IL12 ELISA试剂盒

IL12 (Interleukin 12 (IL12))

别名

IL-12(p70)

基因ID

16159

UniProt

F8WI71

途径

JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome

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