IL-10 ELISA 试剂盒
Quick Overview for IL-10 ELISA 试剂盒 (ABIN2042517)
抗原
See all IL-10 (IL10) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 15.6 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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96-well plate pre-coated with anti-IL-10, Mouse
Lyophilized recombinant IL-10, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse IL-10 in cell culture supernates and serum.Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- IL-10
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基因ID
- 16153
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UniProt
- P18893
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原 See all IL-10 (IL10) ELISA试剂盒
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