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IL-10 ELISA 试剂盒

This 小鼠 IL-10 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 IL-10.
产品编号 ABIN2042517
发货至: 中国

Quick Overview for IL-10 ELISA 试剂盒 (ABIN2042517)

抗原

See all IL-10 (IL10) ELISA试剂盒
IL-10 (IL10) (Interleukin 10 (IL10))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    15.6 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 1 pg/mL

    组件

    96-well plate pre-coated with anti-IL-10, Mouse
    Lyophilized recombinant IL-10, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Mouse IL-10 in cell culture supernates and serum.Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18kD.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all IL-10 (IL10) ELISA试剂盒

    IL-10 (IL10) (Interleukin 10 (IL10))

    别名

    IL-10

    基因ID

    16153

    UniProt

    P18893

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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