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EGF ELISA 试剂盒

This 小鼠 EGF ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 EGF.
产品编号 ABIN2042444
发货至: 中国

Quick Overview for EGF ELISA 试剂盒 (ABIN2042444)

抗原

See all EGF ELISA试剂盒
EGF (Epidermal Growth Factor (EGF))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    15.6 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 1 pg/mL

    组件

    96-well plate pre-coated with anti-EGF, Mouse
    Lyophilized recombinant EGF, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Mouse EGF in cell culture supernates, serum, plasma (heparin, EDTA), tissue homogenates and urine.Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival.1 It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin.2 EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.3

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all EGF ELISA试剂盒

    EGF (Epidermal Growth Factor (EGF))

    别名

    EGF

    基因ID

    13645

    UniProt

    P01132

    途径

    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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